{"id":9149,"date":"2018-10-17T17:43:49","date_gmt":"2018-10-17T21:43:49","guid":{"rendered":"http:\/\/biolinecorp.ca\/?p=9149"},"modified":"2018-10-25T14:32:57","modified_gmt":"2018-10-25T18:32:57","slug":"new_iso_standard_hydrophobic_fulvic_","status":"publish","type":"post","link":"https:\/\/biolinecorp.ca\/pt\/2018\/10\/17\/new_iso_standard_hydrophobic_fulvic_\/","title":{"rendered":"A nova norma internacional aprovada pela ISO para a quantifica\u00e7\u00e3o dos h\u00famicos"},"content":{"rendered":"<p>\u00c1cido h\u00famico e \u00e1cido f\u00falvico s\u00e3o termos que n\u00e3o se referem a um composto qu\u00edmico discreto ou mol\u00e9cula. N\u00e3o h\u00e1 f\u00f3rmula qu\u00edmica definida ou espec\u00edfica, peso molecular ou estrutura para \u00e1cido f\u00falvico ou h\u00famico. Assim, um termo mais preciso a utilizar \u00e9 o plural de \u00e1cidos f\u00falvicos e \u00e1cidos h\u00famicos. O termo \u00e1cido f\u00falvico refere-se a uma grande classe de v\u00e1rias centenas de v\u00e1rios complexos que t\u00eam diferen\u00e7as f\u00edsicas e qu\u00edmicas fundamentais que distinguem os complexos de \u00e1cido h\u00famico dos complexos de \u00e1cido h\u00famico. Existe uma grande varia\u00e7\u00e3o dentro dos complexos de \u00e1cido f\u00falvico ou complexos de \u00e1cido h\u00famico de diferentes misturas de m\u00faltiplas mol\u00e9culas, pesos moleculares e grupos funcionais.<\/p>\n<p>A defini\u00e7\u00e3o mais amplamente aceite de \u00e1cido f\u00falvico \u00e9 a frac\u00e7\u00e3o de subst\u00e2ncias h\u00famicas sol\u00faveis tanto em condi\u00e7\u00f5es \u00e1cidas como alcalinas. Enquanto que os \u00e1cidos h\u00famicos s\u00e3o as frac\u00e7\u00f5es que s\u00e3o sol\u00faveis apenas em condi\u00e7\u00f5es alcalinas. Os \u00e1cidos f\u00falvicos t\u00eam: 1) menor tamanho, 2) menor peso molecular, 3) maior teor de oxig\u00e9nio, 4) maior reactividade qu\u00edmica, e 5) maior actividade microbiana em compara\u00e7\u00e3o com os \u00e1cidos h\u00famicos.<\/p>\n<p>N\u00e3o existe um m\u00e9todo directo para caracterizar ou quantificar o \u00e1cido f\u00falvico ou h\u00famico. Todas as tentativas de caracterizar ou quantificar at\u00e9 \u00e0 data s\u00e3o estimativas, e como tal um m\u00e9todo padr\u00e3o tem sido dif\u00edcil de desenvolver. As primeiras tentativas de quantifica\u00e7\u00e3o utilizaram m\u00e9todos colorim\u00e9tricos (Mehlich, 1984) para quantificar produtos de \u00e1cido h\u00famico e \u00e1cido h\u00famico f\u00falvico, comparando a intensidade da cor com quantidades padr\u00e3o para o \u00e1cido h\u00famico Aldrich. Investigadores demonstraram que diferentes lotes de amostras de \u00e1cido h\u00famico t\u00eam diferentes medi\u00e7\u00f5es colorim\u00e9tricas. Al\u00e9m disso, o m\u00e9todo colorim\u00e9trico faz<\/p>\n<p>n\u00e3o distinguir o f\u00falvico do h\u00famico. Assim, um produto f\u00falvico que tenha mesmo pequenas quantidades de contaminantes h\u00famicos ser\u00e1 de cor mais escura e como tal relatar\u00e1 leituras colorim\u00e9tricas mais elevadas do que uma amostra f\u00falvica mais pura. Outros contaminantes org\u00e2nicos e inorg\u00e2nicos tamb\u00e9m ter\u00e3o impacto na cor, e se presentes na amostra ser\u00e3o medidos e reportados como h\u00famicos ou f\u00falvicos. Fragmentos e derivados de lignina, um pol\u00edmero org\u00e2nico muito comum nas plantas, s\u00e3o tamb\u00e9m castanhos-escuros. A lignina \u00e9 solubilizada e removida da madeira e outra biomassa em opera\u00e7\u00f5es de celulose e papel. As \u00e1guas residuais da pasta e do papel cont\u00eam frequentemente altas concentra\u00e7\u00f5es de lignina, e derivados da lignina que ser\u00e3o indistingu\u00edveis por este m\u00e9todo de subst\u00e2ncias h\u00famicas. Finalmente, ao utilizar este m\u00e9todo, os produtores poderiam reivindicar 30% ou uma concentra\u00e7\u00e3o de \u00e1cido f\u00falvico em solu\u00e7\u00f5es, mas ser\u00e1 que isso significa alguma coisa? O que \u00e9 que o cliente est\u00e1 realmente a receber? Sem uma an\u00e1lise mais detalhada, poder\u00e1 realmente saber?<\/p>\n<p>O <a href=\"https:\/\/www.cdfa.ca.gov\/\"><b><i>Departamento de Alimenta\u00e7\u00e3o e Agricultura da Calif\u00f3rnia (CDFA)<\/i><\/b><\/a> interveio para criar um teste padr\u00e3o para a quantifica\u00e7\u00e3o do \u00e1cido h\u00famico. O m\u00e9todo \u00e9 mais preciso do que o colorim\u00e9trico e pode resultar em concentra\u00e7\u00f5es mais consistentes. Para amostras t\u00edpicas, a quantifica\u00e7\u00e3o usando o m\u00e9todo CDFA resultou frequentemente em concentra\u00e7\u00f5es inferiores a metade do que foi medido usando o m\u00e9todo colorim\u00e9trico. Em 1990, J.M Verploegh e L.A Brandvold publicaram passos adicionais ao CDFA que permitiriam tamb\u00e9m a quantifica\u00e7\u00e3o da frac\u00e7\u00e3o \u00e1cida f\u00falvica. O m\u00e9todo n\u00e3o contabiliza a influ\u00eancia do conte\u00fado de cinzas devido \u00e0 presen\u00e7a de inorg\u00e2nicos como o pot\u00e1ssio (K), s\u00f3dio (Na), e c\u00e1lcio (Ca). Al\u00e9m disso, mais uma vez qualquer esp\u00e9cie org\u00e2nica insol\u00favel em \u00e1cido, como a Klasson Lignin, que constitui a maioria da lignina na biomassa, seria tamb\u00e9m insol\u00favel em \u00e1cido e contribuiria para os n\u00edveis de \u00e1cido h\u00famico quantificados pelo m\u00e9todo CDFA. Por outro lado, os compostos org\u00e2nicos mais comuns como a\u00e7\u00facares, polissac\u00e1ridos, amino\u00e1cidos, prote\u00ednas, hidratos de carbono e outros \u00e1cidos org\u00e2nicos s\u00e3o sol\u00faveis em solu\u00e7\u00f5es \u00e1cidas, e como tal, se estivessem presentes na amostra seriam quantificados como \u00e1cido f\u00falvico por este m\u00e9todo.<\/p>\n<p>Foram as preocupa\u00e7\u00f5es em torno das insufici\u00eancias destes m\u00e9todos que conduziram o <a href=\"http:\/\/www.humictrade.org\/\"><b><i>Associa\u00e7\u00e3o Comercial de Produtos H\u00famicos (HPTA)<\/i><\/b><\/a> para embarcar no desenvolvimento de um novo m\u00e9todo padronizado. Ap\u00f3s oito anos de investiga\u00e7\u00e3o dedicada pela HPTA, a organiza\u00e7\u00e3o conseguiu estabelecer um padr\u00e3o internacional para a an\u00e1lise de \u00e1cidos h\u00famicos e \u00e1cidos f\u00falvicos hidrof\u00f3bicos em produtos h\u00famicos comerciais. A nova norma internacional (<a href=\"https:\/\/www.iso.org\/standard\/66271.html\"><b><i>ISO 19822:2018<\/i><\/b><\/a>) foi publicado pela <a href=\"https:\/\/www.iso.org\/home.html\"><b><i>Organiza\u00e7\u00e3o Internacional de Normaliza\u00e7\u00e3o (ISO)<\/i><\/b><\/a> em Agosto de 2018. A partir de 2014, o m\u00e9todo j\u00e1 tinha sido aprovado pela <a href=\"http:\/\/www.aapfco.org\/\"><b><i>Associa\u00e7\u00e3o de Oficiais Americanos de Controlo Alimentar Vegetal (AAPFCO)<\/i><\/b><\/a>.<\/p>\n<p>O novo m\u00e9todo padr\u00e3o ainda pode ser suscept\u00edvel \u00e0 presen\u00e7a de Klasson lignina, ou outra mat\u00e9ria org\u00e2nica insol\u00favel em \u00e1cido para a quantifica\u00e7\u00e3o do \u00e1cido h\u00famico, no entanto, \u00e9 uma grande melhoria uma vez que remove a influ\u00eancia das cinzas na quantifica\u00e7\u00e3o do \u00e1cido h\u00famico. Al\u00e9m disso, o m\u00e9todo ISO s\u00f3 pode ser utilizado para quantificar os 30 - 50% (por peso) de complexos de \u00e1cido f\u00falvico que exibem interac\u00e7\u00f5es hidrof\u00f3bicas. Os complexos individuais de \u00e1cido f\u00falvico cont\u00eam moieties hidrof\u00f3bicas e hidrof\u00edlicas. Como resultado, o comportamento de um complexo de \u00e1cido f\u00falvico \u00e9 uma fun\u00e7\u00e3o da probabilidade. Os complexos de \u00e1cido f\u00falvico que t\u00eam uma maior concentra\u00e7\u00e3o de locianatos hidrof\u00f3bicos t\u00eam uma maior tend\u00eancia a exibir comportamento hidrof\u00f3bico. Al\u00e9m disso, os lignossulfonatos (derivados comuns da lignina produzida pela produ\u00e7\u00e3o de pasta e papel) tamb\u00e9m se comportam hidrofobicamente e seriam indistingu\u00edveis do \u00e1cido f\u00falvico por este m\u00e9todo. Por esta raz\u00e3o, o novo m\u00e9todo ISO prop\u00f5e-se testar a presen\u00e7a de enxofre, e se forem encontradas concentra\u00e7\u00f5es de enxofre superiores a 0,75%, caracteriza\u00e7\u00e3o posterior usando <a href=\"https:\/\/en.wikipedia.org\/wiki\/Fourier-transform_infrared_spectroscopy\"><b><i>Espectroscopia de Infravermelhos de Transforma\u00e7\u00e3o Fournier (FTIR)<\/i><\/b><\/a> an\u00e1lise a ser utilizada para confirmar a aus\u00eancia de lignosulfonatos.<\/p>\n<p>Embora tenha as suas limita\u00e7\u00f5es, o novo m\u00e9todo ISO \u00e9 o melhor m\u00e9todo que temos encontrado at\u00e9 \u00e0 data que aborda a infla\u00e7\u00e3o da quantidade de \u00e1cido f\u00falvico reportada pelos inorg\u00e2nicos, bem como pela maioria dos aditivos org\u00e2nicos n\u00e3o f\u00falvicos ou adulterantes. Como membro da Associa\u00e7\u00e3o Comercial de Produtos H\u00famicos (HPTA), apoiamos vivamente o desenvolvimento de um novo m\u00e9todo padr\u00e3o destinado a permitir um meio mais preciso de medir e comparar os produtos no mercado.<\/p>\n<p>Visite <a href=\"http:\/\/www.humictrade.org\/wp-content\/uploads\/2018\/07\/ISO-Report-July-2018.pdf\">http:\/\/www.humictrade.org<\/a> para mais informa\u00e7\u00f5es.<\/p>","protected":false},"excerpt":{"rendered":"<p>Humic and fulvic acid are terms that do not refer to a discrete chemical compound or molecule. There is no defined or specific chemical formula, molecular weight or structure for fulvic or humic acid. Thus, a more accurate term to use is the plural fulvic acids and humic acids. The term fulvic acid is referring [&hellip;]<\/p>","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[408],"tags":[],"class_list":["post-9149","post","type-post","status-publish","format-standard","hentry","category-biostimulants"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v24.5 (Yoast SEO v24.5) - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>New ISO Standard for quantification of hydrophobic fulvic acid - BioLiNE<\/title>\n<meta name=\"description\" content=\"The new ISO standard (ISO 19822:2018) approved. The HPTA was successful in establishing an international standard for hydrophobic fulvic acid quantification\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/biolinecorp.ca\/pt\/2018\/10\/17\/new_iso_standard_hydrophobic_fulvic_\/\" \/>\n<meta property=\"og:locale\" content=\"pt_PT\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"The New ISO Approved International Standard for Quantification of Humics\" \/>\n<meta property=\"og:description\" content=\"The new ISO standard (ISO 19822:2018) approved. The HPTA was successful in establishing an international standard for hydrophobic fulvic acid quantification\" \/>\n<meta property=\"og:url\" content=\"https:\/\/biolinecorp.ca\/pt\/2018\/10\/17\/new_iso_standard_hydrophobic_fulvic_\/\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/BiolineCorp\/\" \/>\n<meta property=\"article:published_time\" content=\"2018-10-17T21:43:49+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2018-10-25T18:32:57+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/biolinecorp.ca\/wp-content\/uploads\/2018\/10\/HPTA-Certified-Bug-4C-CIRCLE-R.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"3600\" \/>\n\t<meta property=\"og:image:height\" content=\"3600\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Mohammad Rahbari\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@BiolineCorp\" \/>\n<meta name=\"twitter:site\" content=\"@BiolineCorp\" \/>\n<meta name=\"twitter:label1\" content=\"Escrito por\" \/>\n\t<meta name=\"twitter:data1\" content=\"Mohammad Rahbari\" \/>\n\t<meta name=\"twitter:label2\" content=\"Tempo estimado de leitura\" \/>\n\t<meta name=\"twitter:data2\" content=\"5 minutos\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/biolinecorp.ca\/2018\/10\/17\/new_iso_standard_hydrophobic_fulvic_\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/biolinecorp.ca\/2018\/10\/17\/new_iso_standard_hydrophobic_fulvic_\/\"},\"author\":{\"name\":\"Mohammad Rahbari\",\"@id\":\"https:\/\/biolinecorp.ca\/#\/schema\/person\/8e3de19a4e8f3823c087cd0cb0f794dc\"},\"headline\":\"The New ISO Approved International Standard for Quantification of Humics\",\"datePublished\":\"2018-10-17T21:43:49+00:00\",\"dateModified\":\"2018-10-25T18:32:57+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/biolinecorp.ca\/2018\/10\/17\/new_iso_standard_hydrophobic_fulvic_\/\"},\"wordCount\":948,\"publisher\":{\"@id\":\"https:\/\/biolinecorp.ca\/#organization\"},\"articleSection\":[\"Biostimulants\"],\"inLanguage\":\"pt-PT\"},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/biolinecorp.ca\/2018\/10\/17\/new_iso_standard_hydrophobic_fulvic_\/\",\"url\":\"https:\/\/biolinecorp.ca\/2018\/10\/17\/new_iso_standard_hydrophobic_fulvic_\/\",\"name\":\"New ISO Standard for quantification of hydrophobic fulvic acid - BioLiNE\",\"isPartOf\":{\"@id\":\"https:\/\/biolinecorp.ca\/#website\"},\"datePublished\":\"2018-10-17T21:43:49+00:00\",\"dateModified\":\"2018-10-25T18:32:57+00:00\",\"description\":\"The new ISO standard (ISO 19822:2018) approved. 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